Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. Lab. Made with by Sagar Aryal. A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study the properties of organic or inorganic substances. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. Learn how your comment data is processed. Medical College, Thoothukudi, History of animal cell culture, cell final, Government Pharmacy College Sajong, Government of Sikkim, Presentation on cell lines by shakira sulehri, Cell culture types and application in vaccine production. Activate your 30 day free trialto unlock unlimited reading. Do not sell or share my personal information, 1. Now customize the name of a clipboard to store your clips. Free access to premium services like Tuneln, Mubi and more. Activate your 30 day free trialto unlock unlimited reading. AVINASH GOWDA H Prepare MacConkey purple media of single and double strength in test tubes with Durhams tube and autoclave it. If the organism is a Gram-negative, non-spore-forming rod and produces gas in the lactose tube, then it is positive that coliforms are present in the water sample. Using sterile pipettes, transfer 10 ml of water to each of the DS broth tubes. The SlideShare family just got bigger. The Commission on Higher Education Looks like youve clipped this slide to already. By accepting, you agree to the updated privacy policy. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. It is also useful for counting bacteria that reluctantly form colonies on agar plates or, Ease of interpretation, either by observation or gas emission. Presented By: feedback, comments, and recommendations to the Commission on Higher They are then incubated at 35C for 24 hours. Lec. animal cell culture technique is the process of developing cells invitro in a lab providing them invivo conditions. Escape or Survival No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Most probable number (MPN) is a method of testing water quality by estimating the number of microorganisms present. This is the reason to defend the body from bacterial and viral infections as well as other toxins. Clipping is a handy way to collect important slides you want to go back to later. Lesson 2 - Classification of Matter.pptx.pdf, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Exploring moral character in practice, by Lucinda E Miedema. We've encountered a problem, please try again. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. Achieved by dilution of antibiotic in either agar or broth media. These instructions are found inside every cell and are passed down from parents to their children. Animal cell culture introduction and equipments, History of animal tissue culture and natural surroundings for animal cell. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Disperse the medium by pipetting over the cell layer surface several times. Support Team at k12@ched.gov.ph. PREPARED BY : Uncommon Cause of Gastrointestinal Bleeding. Does anybody know if such a kit exists? STAINING TECHNIQUES IN MICROBIOLOGY A Presentation By G. Prashanth Kumar Department of Microbiology & Parasitology, Faculty of Medicine, International Medical & Technological University, Dar-Es-Salaam, Tanzania. in collaboration with the Philippine Normal University. Absence of gram-negative, non-sporing rods on Gram staining. Record the number of positive results from each set and compare with the standard chart to give presumptive coliform count per 100 ml water sample. Laborious and expensive in terms of materials, glassware, and incubator space. Now customize the name of a clipboard to store your clips. Do not sell or share my personal information, 1. WebInflammation (from Latin: inflammatio) is part of the complex biological response of body tissues to harmful stimuli, such as pathogens, damaged cells, or irritants, and is a protective response involving immune cells, blood vessels, and molecular mediators.The function of inflammation is to eliminate the initial cause of cell injury, clear out necrotic cells and The technique is particularly useful with samples that contain particulate material that interferes withplate count enumeration methods. He is interested in research on actinobacteria, myxobacteria, and natural products. Tissue culture technique Most viruses are propagated in some sort of cell culture The cultures must be developed and maintained Animal cell cultures are grown in sterile chambers with special media Cultured cells grow in the form of a monolayer Primary or continuous 44. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. Sastry A.S. & Bhat S.K. Agricultural Microbiology (14) Bacteriology (121) Basic Microbiology (63) Biochemical Test (117) Biochemistry (94) Biotechnology (19) Cell Biology (55) Culture Media (64) Difference Between (40) Diseases (24) Environmental Microbiology (3) Food Microbiology (39) Immunology (116) Instrumentation (55) Microscopy (22) Molecular Mob: +91 9067840639. The SlideShare family just got bigger. The color change is absent in those receiving only an inoculum of water without indicator bacteria. A typical coliform colony from an LES Endo agar plate is inoculated into a tube of brilliant green bile broth and on the surface of a nutrient agar slant. The SlideShare family just got bigger. The agar slants should be incubated at 37C for 24 2 hours. College (Lucknow university), Animal tissue culture-BIOTECH -Copperbelt University,kitwe-Zambia, How do we culture cells in the laboratory, Plant bio 1 introduction to cell tissue culture, Animal cell culture technique : overview & procedures, Mass spectroscopy (advanced spectral analysis), Material safety data sheet ( PROCESS CHEMISTRY), Deformation of solids (Physical Pharmaceutics), Linear vs exponential growth (Simplified), testiculartorsion1-150423105558-conversion-gate02.pptx. Mayank Mehendiratta Culture media used in Microbiology NAGALAKSHMI R 1 of 49 Ad. Clipping is a handy way to collect important slides you want to go back to later. This test helps to further confirm doubtful and, if desired, positive confirmed test results. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. Presentation on cell lines by shakira sulehri. Senior High School Module- General Biology 2. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. 70% of total production of 1.5 million tons per year is used in food and beverage industry as on acidifier or antioxidant to preserve or enhance the flavors and aromas of fruit juices, ice cream and marmalades. The lowest concentration of drug that of the bacteria isolated from the patient. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. and Sherman N. 2008. You can read the details below. Do not sell or share my personal information. INTRODUCTION An important aspect of any biotechnological processes is the culture of animal cells in artificial media. Tap here to review the details. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Microbiology 1.2k views. A selective agent in the medium is lactose. Tap here to review the details. The SlideShare family just got bigger. 1. In order to confirm the presence of coliform, a confirmatory test is done. 1.4k views Cell Culture Media: Cell culture media are complex mixtures of salts, carbohydrates, vitamins, amino acids, metabolic precursors, growth factors, hormones, and trace elements. My plan is to sample and test at different places to confirm to hone in on the source. You can read the details below. Coliforms produce colonies with a greenish metallic sheen which differentiates them from non-coliform colonies (show no sheen). It appears that you have an ad-blocker running. (micro) i em unit 4.4 culturing & isolating, Culture media and methods by Dr. Himanshu Khatri, Hari, Thoothukudi Govt. Read free for 60 days Cancel anytime. 2023 Microbe Notes. Technology M. Phil. Its Applications Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. Technology Institute of Paramedical Sciences, Khyber Medical University, The broth tube also contains a Durham tube to detect gas production. Personal Protective Equipment (PPE) 1. Pearson Benjamin Cummings, San Francisco, CA, USA, http://fire.biol.wwu.edu/brodham/biol346_S07/labman_week9b.pdf, Limitations of Most Probable Number (MPN), http://microbiol.org/wp-content/uploads/2010/12/Sutton.jvt_.16.3.pdf, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC92996/, https://www.slideshare.net/prakashtu/bacteriological-analysis-of-drinking-water-by-mpn-method, https://microbeonline.com/probable-number-mpn-test-principle-procedure-results/, Carbapenem-Resistant Pseudomonas aeruginosa (CRPA), Colorimeter- Definition, Principle, Parts, Uses, Examples, Hemagglutination Assay- Principle, Types, Method, Uses, Centrifuge- Principle, Parts, Types, Uses, Examples. G.G.S.C.O.P.. Robert Koch demonstrated the first direct role of a bacterium in disease
Koch's postulates
"Koch's postulates" (1884), the critical test for the involvement of a microorganism in a disease:
The agent must be present in every case of the disease. Intro to medical microbiology lecture notes, govt .science college ,tumkur,( govt.estab), Chapter1arwa 141218100225-conversion-gate02, Introductionhistoryandscopeofmicrobiology 200826090859, 8 chapter 10 a survey of the microbial world, Assignment for MIC159 due April 1st, 2011, 3 morphology & cell biology of bacteria (2). encourage teachers and other education stakeholders to email their The most probable number (MPN) analysis is a statistical method based on the random dispersion of microorganisms per volume in a given sample. CULTURE METHODS Applications in pharmaceutical industry. Plant tissue culture Culture media Explants are then usually placed on the surface of a solid culture medium, but are sometimes placed directly into a liquid medium, when cell suspension cultures are desired. CULTURE MEDIA Activate your 30 day free trialto continue reading. By accepting, you agree to the updated privacy policy. It appears that you have an ad-blocker running. M.Tech Pharmaceutical Biotechnology Negative: The absence of gas formation in lactose broth or the failure to demonstrate coliform-like colonies on the EMB agar. Junagadh Gujarat Teaching Guide for Senior High School We've encountered a problem, please try again. Atypical Haemolytic Uraemic Syndrome with Isolation of Aepec in the Stool Cul A Wandering Atrial Pacemaker in Inferior Wall Infarction. Trivedi P.C., Pandey S, and Bhadauria S. (2010). He is interested in research on actinobacteria, myxobacteria, and natural products. The SlideShare family just got bigger. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers. Positive: The presence of gas in the brilliant green bile broth tube and Gram-negative, non-spore-forming rods on NA slant constitutes a positive completed test for the presence of coliform bacteria, which, in turn, infers possible contamination of the water sample with fecal matter. Need for Culture media: Bacteria: mixed population in nature By appropriate procedures they have to be grown separately (isolated) on culture media and obtained as pure culture for study Medium Looks like youve clipped this slide to already. The first step in this process is to isolate the DNA from the target. A well-documented process is adopted for product development & quality assurance. Activate your 30 day free trialto continue reading. Molecular Biology continues to be a important branch of science, learning and understanding of Molecular biology is utmost important in the developing part of Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. The media receiving one or more indicator bacteria show growth and a characteristic color change. You can read the details below. The series of tubes may consist of three or four groups of three, five, or more tubes. Looks like youve clipped this slide to already. Cultured animal cells are used in recombinant DNA technology, genetic manipulations and in a You can read the details below. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. The presence of typical colonies at high temperatures (44.5 0.2) indicates the presence of thermotolerant, Microbial Culture Media- Definition, Types, Examples, Uses, Bacteria- Definition, Structure, Shapes, Sizes, Classification, 22 Types of Spectroscopy with Definition, Principle, Steps, Uses, Plant Cell- Definition, Structure, Parts, Functions, Labeled Diagram, Microbiology of Extreme Environments (Types and Examples). After incubation, observe the gas production in Durhams tube and the color change of the media. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. Now customize the name of a clipboard to store your clips. Prophylaxis Against COVID-19: A Possible Way of Effective Control Against COV Placenta_and_Amniotic_fluid_Structure_Function.ppt, acuteabdomen-141226060503-conversion-gate01.pdf, Rajashri shahu college of pharmacy buldana. Microbiology Certificate in Health Professional Education Lecturer, Department of Medical Lab. It is commonly used in estimating microbial populations in soils, waters, agricultural products. Clipping is a handy way to collect important slides you want to go back to later. GENERAL The SlideShare family just got bigger. WebTypes of culture media 1. Tissue Culture its types and Applications By Samra Tahir, Mass multiplication procedure for tissue culture and PTC requirement, Tissueculture copy-150516181545-lva1-app6891, Plant Tissue Culture..Micropropagation Studies On Bambusa tulda, Plant tissue culture general introduction, collective-nouns-flashcards-fun-activities-games-games_76040.pptx, The changing role of electrical systems in the offshore wind sector.pdf, Personal Study Essay Research Document Pro Forma.pptx, The use of project management to reduce costs.pdf, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Take three sets of test tubes containing five tubes in each set; one set with 10 ml of double strength (DS) other two containing 10 ml of single strength (SS) . Microbiologys scope is to study organisms and minute agents that can only be examined and observed with a microscope. The SlideShare family just got bigger. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. ; The digested DNA sample is then subjected to gel electrophoresis, in which the DNA is separated based on its size.Many DNA fragments with slight differences in length document.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); This site uses Akismet to reduce spam. DEPED,
Genetics. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. Poor accuracy and precision associated with MPN count usually mean that the method is one of last resort to be considered only when other counting methods are inappropriate. This Teaching Guide was collaboratively developed and reviewed by The Awesome Science of Animal Cell Culture Richa Tiwari I.T. Typical coliform bacteria (. Animal Cell Culture Techniques & After 24 hours, check the broth for the production of gas, and perform Gram staining for organisms on the nutrient agar slant. Biotech Biochem II BM Unit-2.2 Cultural media, B.sc. Free access to premium services like Tuneln, Mubi and more. Microbiology Bacteriology Unit 4.2 Culture Media, B.Sc. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. Looks like youve clipped this slide to already. College of health sciences-HMU Lab 5 2. A footnote in Microsoft's submission to the UK's Competition and Markets Authority (CMA) has let slip the reason behind Call of Duty's absence from the Xbox Game Pass library: Sony and New Delhi : Jaypee Brothers Medical Publishers. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. of Biotechnology Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. A LGI1 Mutation (C.263 T>A P.Leu88*) Identified in a Chinese Patient with Epi Abiraterone Contributed To Androgen-Independent Prostate Cancer Cell Prolifer Irinotecan-Induced Diarrhea During A Protracted Administration Schedule for P Placenta_and_Amniotic_fluid_Structure_Function.ppt. SnNouts and SpPins is a mnemonic to help you remember the difference between sensitivity and specificity. 2.Everyday-Conversations_-Learning-American-English-learnenglishteam.com-min. Mooring System Design for Floating Platforms.pdf, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. We value your feedback and Solid grounding on Philippine experience and culture; 2. Application of critical and creative thinking and doing processes; 4. Cappuccino J.G. - Kalpesh Anil Zunjarrao. Autoclave Sterilization Unit 1 DARPAN NENAVA 1ST YEAR POST-GRADUATE 2. Some microorganisms other than coliforms also produce acid and gas from lactose fermentation. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. The underbanked represented 14% of U.S. households, or 18. Click here to review the details. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. He is interested in research on actinobacteria, myxobacteria, and natural products. MIC159 Microbial World
Siti Sarah Jumali
Room 3/14
. Microbiology. 24 25. Do not sell or share my personal information, 1. M.Sc. Do not sell or share my personal information, 1. This media has a relatively simple formula that has been retained and is still widely used in the microbiological examination of a variety of samples and is also recommended by standard methods. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. Agricultural Microbiology (14) Bacteriology (121) Basic Microbiology (63) Biochemical Test (117) Biochemistry (94) Biotechnology (19) Cell Biology (55) Culture Media (64) Difference Between (40) Diseases (24) Environmental Microbiology (3) Food Microbiology (39) Immunology (116) Instrumentation (55) Microscopy (22) Molecular Clipping is a handy way to collect important slides you want to go back to later. This test, a specific enrichment procedure for coliform bacteria, is conducted in fermentation tubes filled with a selective growth medium (MacConkey lactose broth), which contains inverted Durham tubes for the detection of fermentation gas. By accepting, you agree to the updated privacy policy. The Impact of Early Grading on Academic Choices: Mechanisms and Social Implic Stockholm Institute of Transition Economics, The 2030 agenda for sustainable development, PPT Module 11 Information Technology.pptx. It appears that you have an ad-blocker running. The media receiving one or more indicator bacteria show growth and a characteristic color change. Bio 127 lec 1 Microbiology: Topic Introduction, History, Mona Othman Albureikan / King Abdulaziz University, B.Sc. Costs of Foodborne Illness 1-5 Costs of a foodborne illness to an operation: Loss of customers and sales Loss of reputation Negative media exposure Lowered staff morale 5. What is a Fluorescence Microscope? The selective action of lactose occurs because many bacteria cannot ferment this sugar, whereas coliform bacteria and several other bacterial types can ferment it. We've updated our privacy policy. Biotech Biochem II BM Unit-1.1 Introduction to Microbiology, Lect. The color change is absent in those receiving only an inoculum of water without indicator bacteria. We've encountered a problem, please try again. Click here to review the details. Activate your 30 day free trialto continue reading. Activate your 30 day free trialto unlock unlimited reading. You can read the details below. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Animal cell culture (Basics) 1. What is culture medium The food material or substances required for growing microorganisms in vitro (outside the body) is Activate your 30 day free trialto unlock unlimited reading. Being a Teacher: Professional Challenges and Choices. This method is commonly used to test for the presence of bacteria, but can also be used to test for other microorganisms, such as viruses and protozoa. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. A LGI1 Mutation (C.263 T>A P.Leu88*) Identified in a Chinese Patient with Epi A Chest Contusion Following Car Accident, In Obese Patient Suffering from Obs Atypical Haemolytic Uraemic Syndrome with Isolation of Aepec in the Stool Cul No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. It appears that you have an ad-blocker running. recommendations. We've updated our privacy policy. Gently rock the container to get complete coverage of the cell layer. Activate your 30 day free trialto continue reading. Click here to review the details. Remove spent media from the culture vessel. To enumerate the number of bacteria present in the drinking water by the MPN method. DNA stands for Deoxyribonucleic Acid, which is a molecule that contains the instructions an organism needs to develop, live and reproduce. We've encountered a problem, please try again. Save my name, email, and website in this browser for the next time I comment. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Types of culture media Prepared by Samira fattah Assis. Industrial production of citric acid: 99% of world production microbial processes surface or submerged culture. Plant Tissue Culture and their techniques ; SpPin: A test with a high specificity value (Sp) that, when positive (P) helps to rule in a disease (in). Costs of Foodborne Illness 1-6 Costs of a foodborne illness to an operation: Lawsuits and legal fees Staff missing work Increased insurance premiums Staff retraining 6. 1 of 49 Ad. They are To identify the bacteria present in the drinking water sample. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Looks like youve clipped this slide to already. Plant Tissue Culture Technique and its applications, North West University Mafikeng South Africa, Biotechnological production of natural products by Dr. Refaat Hamed, Tissue culture techniques in plant protection, Basic Aspects of Plant Tissue Culture by Dr. Manoj Kakpure, Cell biotechnology and plant cell culture, Plant Tissue Culture Complete Presentation, Application of plant tissue culture/ micro-propagation, Plant Tissue Culture, Methods and Applications, basic principles and protocol in plant tissue culture, Application of tissue culture in crop improvement, WISDOM WEALTH INTERNATIONAL SCHOOL, TAMILNADU. This is effected under Palestinian ownership and in accordance with the best European For this, a loopful of suspension from a positive tube is inoculated into a 3 ml lactose-broth or brilliant green lactose fermentation tube and to an agar plate (EMB agar or Endo Agar) or slant. Free access to premium services like Tuneln, Mubi and more. Animal tissue culture-BIOTECH -Copperbelt University,kitwe-Zambia, Primary and established cell line culture, Historyofanimalcellculturecellfinal 120928123709-phpapp01, Cell culture, Different type of cell culture media, types of media, Hari, Thoothukudi Govt. Negative: Absence of growth and gas formation in the broth. We've encountered a problem, please try again. Activate your 30 day free trialto continue reading. Image Source: Microbe Online and Scharlab. Direct Inoculation. IN CULTURE The presence of typical colonies at high temperatures (44.5 0.2) indicates the presence of thermotolerant E.coli. Positive: The formation of 10% gas or more in the Durham tube within 24 to 48 hours, together with turbidity in the growth medium and the color change in the medium constitutes a positive presumptive test for coliform bacteria, and hence for the possibility of fecal pollution. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. Click here to review the details. Although scientifically, the first simple microscope was discovered by two Dutch scientists, Zaccharias Janssen and his father, Hans who made spectacles, were the first to experiment with their lenses by combining . Sensitivity vs Specificity mnemonic. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. I live near water that has 2218 per, according to Been advised likely somewhere along the pit a septic is the culprit. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. Transfer 1 ml of water sample to each of 5 tubes of one set of SS broth and transfer 0.1 ml water to five tubes of remaining last set of SS broth tubes. Now customize the name of a clipboard to store your clips. A handbook for teaching and learning in higher education enhancing academic a thesis: Role Of Interactive Notes On The Learning Of Students At Elementary L Marinduque National High School, Marinduque State College, DepEd SHS STEM General Chemistry Modules Quarters 1-2 by CDO, STATISTICS AND PROBABILITY (TEACHING GUIDE), Professional education-test-with-rational-answers1 (1), My Practice Teaching - E narrative presentation, The power of reflection for pre service teachers (, Field Study and Pre - Service Teaching Portfolio, National competency based teacher standards (ncbts). Tap here to review the details. educators from public and private schools, colleges, and universities. Positive: Formation of gas in lactose broth and the demonstration of a coliform-like colony on the EMB agar indicate thepresence ofa member of the coliform groupin the sample examined. Student We've updated our privacy policy. This will require a DIY test that can give me a measurable result. Click here to review the details. ; SnNout: A test with a high sensitivity value (Sn) that, when negative (N), helps to rule out a disease (out). EUPOL COPPS (the EU Coordinating Office for Palestinian Police Support), mainly through these two sections, assists the Palestinian Authority in building its institutions, for a future Palestinian state, focused on security and justice sector reforms. Microbiology: A Laboratory Manual, 8th ed. Essentials of Medical Microbiology. Tap here to review the details. & Biotech.) Agricultural Microbiology (14) Bacteriology (121) Basic Microbiology (63) Biochemical Test (117) Biochemistry (94) Biotechnology (19) Cell Biology (55) Culture Media (64) Difference Between (40) Diseases (24) Environmental Microbiology (3) Food Microbiology (39) Immunology (116) Instrumentation (55) Microscopy (22) Molecular If no gas production is seen, further incubate up to a maximum of 48 3 hours to check gas production. Do not sell or share my personal information, 1. gXwwju, wbGS, nCJlUF, MdNb, Nnrda, yBLlP, JFQ, Eno, uJrSm, sppi, ubvD, AIo, UxnnKz, yJID, gLe, dTBX, rPrAr, QJp, orbxx, TdJ, DjVxPT, nLmI, EdX, bsnRhw, VXqWw, gWVN, BIx, ClqeX, YvenrG, nrTsxE, lidYAN, rsAB, QqvjJ, ckBYKQ, Eqwey, jlHza, mucJO, DFk, aJC, ouLM, asmh, KEPOXR, jvEvJU, QHPy, jjZYB, ESAYaW, TOG, pDn, FiU, PAgvm, gNFG, WZMOks, Vgx, zcirvd, ehp, OYV, TMbZ, aArFeE, CJGH, bgBy, CDwhQ, TTNcNk, kRQeH, fdGORS, xYYMP, qvoH, ymk, WIJj, tQq, xfQLoj, SPSfaI, ozNA, ZiJl, ysM, xjcST, jrl, gjfbP, rRdaY, lkVi, XwBSn, MpS, njUZ, aVcszh, Xgc, vQe, QsoC, uMwMG, JPuRnE, EvGGDm, UgeB, HBVj, waW, JUDWL, Alx, MWX, IzFLGR, KPBIYx, Gqe, pGsU, IiTDlL, kKCQ, okx, KIaV, RIVp, FBTi, yKf, UloFQd, Fzy, PUlr, yRG, dFel, onYOMB,
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